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MDR3cyte

MDR3cyte™ is a patent-pending assay that is the only physiologically relevant assay available to assess test agent inhibition of human multidrug resistance protein 3 (MDR3). MDR3cyte™ is a hepatocyte-based platform that measures test agent inhibition of MDR3, provides cross-species studies, and offers the ability to investigate the effect of in situ drug metabolism on inhibition; the assay is run without radioactive reagents.

MDR3cyte™ employs a 96-well plate format with liquid chromatography-mass spectrometry quantification. The assay is accurate, reproducible, sensitive, and possesses a wide dynamic range. MDR3cyte™ was developed to be cost competitive and high-throughput.

MDR3cyte™ shows excellent correlation with drugs that cause human drug-induced liver injury (DILI) and MDR3 inhibition. MDR3cyte™ is your assay to test MDR3 inhibition and minimize the risk of unexpected DILI.

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Publications:

Aleo MD, Shah F, He K, Bonin PD, Rodrigues AD. Evaluating the Role of Multidrug Resistance Protein 3 (MDR3) Inhibition in Predicting Drug-Induced Liver Injury Using 125 Pharmaceuticals. Chem Res Toxicol. 2017 May 15;30(5):1219-1229. doi: 10.1021/acs.chemrestox.7b00048. Epub 2017 May 4. PubMed PMID: 28437613.http://pubs.acs.org/doi/abs/10.1021/acs.chemrestox.7b00048

Yucha RW, He K, Shi Q, Cai L, Nakashita Y, Xia CQ, Liao M. In Vitro Drug-Induced Liver Injury Prediction: Criteria Optimization of Efflux Transporter IC50 and Physicochemical Properties. Toxicol Sci. 2017 Jun 1;157(2):487-499. doi:10.1093/toxsci/kfx060. PubMed PMID: 28369588. https://academic.oup.com/toxsci/article-abstract/157/2/487/3092345/In-Vitro-Drug-Induced-Liver-Injury-Prediction?redirectedFrom=fulltext

Zhang J, He K, Cai L, Chen YC, Yang Y, Shi Q, Woolf TF, Ge W, Guo L, Borlak J, Tong W. Inhibition of bile salt transport by drugs associated with liver injury in primary hepatocytes from human, monkey, dog, rat, and mouse. Chem Biol Interact. 2016 Mar 19. pii: S0009-2797(16)30089-8. doi:10.1016/j.cbi.2016.03.019. [Epub ahead of print] PubMed PMID: 27000539.http://www.sciencedirect.com/science/article/pii/S0009279716300898

He K, Cai L, Shi Q, Liu H, Woolf TF. Inhibition of MDR3 Activity in Human Hepatocytes by Drugs Associated with Liver Injury. Chem Res Toxicol. 2015 Oct 19;28(10):1987-90. doi: 10.1021/acs.chemrestox.5b00201. Epub 2015 Sep 15. PubMed PMID: 26335978. http://pubs.acs.org/doi/10.1021/acs.chemrestox.5b00201

 


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